Human organomics: a fresh approach to understanding human development using single-cell transcriptomics.
Development
; 144(9): 1584-1587, 2017 05 01.
Article
em En
| MEDLINE
| ID: mdl-28465333
ABSTRACT
Innovative methods designed to recapitulate human organogenesis from pluripotent stem cells provide a means to explore human developmental biology. New technologies to sequence and analyze single-cell transcriptomes can deconstruct these 'organoids' into constituent parts, and reconstruct lineage trajectories during cell differentiation. In this Spotlight article we summarize the different approaches to performing single-cell transcriptomics on organoids, and discuss the opportunities and challenges of applying these techniques to generate organ-level, mechanistic models of human development and disease. Together, these technologies will move past characterization to the prediction of human developmental and disease-related phenomena.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Organoides
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Perfilação da Expressão Gênica
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Crescimento e Desenvolvimento
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Análise de Célula Única
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article