A new role for Rrm3 in repair of replication-born DNA breakage by sister chromatid recombination.
PLoS Genet
; 13(5): e1006781, 2017 May.
Article
em En
| MEDLINE
| ID: mdl-28475600
ABSTRACT
Replication forks stall at different DNA obstacles such as those originated by transcription. Fork stalling can lead to DNA double-strand breaks (DSBs) that will be preferentially repaired by homologous recombination when the sister chromatid is available. The Rrm3 helicase is a replisome component that promotes replication upon fork stalling, accumulates at highly transcribed regions and prevents not only transcription-induced replication fork stalling but also transcription-associated hyper-recombination. This led us to explore the possible role of Rrm3 in the repair of DSBs when originating at the passage of the replication fork. Using a mini-HO system that induces mainly single-stranded DNA breaks, we show that rrm3Δ cells are defective in DSB repair. The defect is clearly seen in sister chromatid recombination, the major repair pathway of replication-born DSBs. Our results indicate that Rrm3 recruitment to replication-born DSBs is crucial for viability, uncovering a new role for Rrm3 in the repair of broken replication forks.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Troca de Cromátide Irmã
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DNA Helicases
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Proteínas de Saccharomyces cerevisiae
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Quebras de DNA de Cadeia Dupla
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article