Cloning and characterization of a genomic probe for malignant catarrhal fever virus.
Am J Vet Res
; 49(10): 1665-8, 1988 Oct.
Article
em En
| MEDLINE
| ID: mdl-2847602
ABSTRACT
A genomic probe specific for malignant catarrhal fever (MCF) virus was cloned by using purified viral DNA from MCF-virus strain WCll. Restriction endonuclease analysis of the purified viral DNA was used to identify the cloned viral genomic fragment. Dot blot hybridization by use of the genomic probe (pRP-5) indicated that the probe hybridized specifically with WCll-MCF virus, as well as with one other isolate of MCF-associated herpesvirus. Hybridization also was observed to a non-MCF virus strain of bovine herpesvirus.
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Base de dados:
MEDLINE
Assunto principal:
DNA Viral
/
Sondas de DNA
/
Herpesviridae
/
Febre Catarral Maligna
Limite:
Animals
Idioma:
En
Ano de publicação:
1988
Tipo de documento:
Article