H. pylori modifies methylation of global genomic DNA and the gastrin gene promoter in gastric mucosal cells and gastric cancer cells.
Microb Pathog
; 108: 129-136, 2017 Jul.
Article
em En
| MEDLINE
| ID: mdl-28478202
ABSTRACT
AIMS:
The aim of this study was to evaluate the correlation between H. pylori infection and global DNA methylation, as well as the methylation levels of the gastrin promoters. MATERIALS ANDMETHODS:
We constructed a eukaryotic expression vector, pcDNA3.1cagA, and transfected it into GES-1 gastric mucosal cells and SGC-7901 gastric cancer cells. Both cell lines were infected with the H. pylori/CagA+ strain NCTC11637. Then, we detected global DNA methylation by capture and detection antibodies, followed by colorimetric quantification. The methylation levels of the gastrin promoter were evaluated by base-specific cleavage and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.RESULTS:
In H. pylori/CagA+-infected GES-1 and SGC-7901 cells, the methylation levels of genomic DNA decreased by 49.4% and 18.8%, and in GES-1 and SGC-7901 cells transfected with pcDNA3.1cagA, the methylation levels of genomic DNA decreased by 17.05% and 25.6%, respectively. Among 24 methylation sites detected in the gastrin promoter region, the methylation levels of 9 CpG sites were significantly decreased in H. pylori/CagA+-infected and pcDNA3.1 cagA-transfected cells in comparison to corresponding control cells.CONCLUSION:
These results indicate that H. pylori/CagA+ decreases the methylation of the genome and the gastrin promoter at some CpG sites in gastric mucosal and gastric cancer cells.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias Gástricas
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Gastrinas
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Helicobacter pylori
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Infecções por Helicobacter
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Regiões Promotoras Genéticas
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Metilação de DNA
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Mucosa Gástrica
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article