Induced accumulation of tyramine, serotonin, and related amines in response to Bipolaris sorokiniana infection in barley.
Biosci Biotechnol Biochem
; 81(6): 1090-1098, 2017 Jun.
Article
em En
| MEDLINE
| ID: mdl-28485206
ABSTRACT
The inducible metabolites were analyzed in barley leaves inoculated with Bipolaris sorokiniana, the causal agent of spot blotch of barley. HPLC analysis revealed that B. sorokiniana-infected leaves accumulated 4 hydrophilic compounds. They were purified by ODS column chromatography and preparative HPLC. Spectroscopic analyses revealed that they were tyramine (1), 3-(2-aminoethyl)-3-hydroxyindolin-2-one (2), serotonin (3), and 5,5'-dihydroxy-2,4'-bitryptamine (4). Among these, 2 and 4 have not been reported as natural products. They showed antifungal activity in an assay of inhibition of B. sorokiniana conidia germination, suggesting that they play a role in the chemical defense of barley as phytoalexins. The accumulation of 1-4 was examined also in the leaves of rice and foxtail millet. Rice leaves accumulated 2, 3, and 4, whereas foxtail millet leaves accumulated 3 and 4 in response to pathogen attack, suggesting the generality of accumulation of 3 and 4 in the Poaceae species.
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Base de dados:
MEDLINE
Assunto principal:
Doenças das Plantas
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Sesquiterpenos
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Esporos Fúngicos
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Hordeum
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Saccharomycetales
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Antifúngicos
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article