Long non-coding RNA PARTICLE bridges histone and DNA methylation.
Sci Rep
; 7(1): 1790, 2017 05 11.
Article
em En
| MEDLINE
| ID: mdl-28496150
ABSTRACT
PARTICLE (Gene PARTICL- 'Promoter of MAT2A-Antisense RadiaTion Induced Circulating LncRNA) expression is transiently elevated following low dose irradiation typically encountered in the workplace and from natural sources. This long non-coding RNA recruits epigenetic silencers for cis-acting repression of its neighbouring Methionine adenosyltransferase 2A gene. It now emerges that PARTICLE operates as a trans-acting mediator of DNA and histone lysine methylation. Chromatin immunoprecipitation sequencing (ChIP-seq) and immunological evidence established elevated PARTICLE expression linked to increased histone 3 lysine 27 trimethylation. Live-imaging of dbroccoli-PARTICLE revealing its dynamic association with DNA methyltransferase 1 was confirmed by flow cytometry, immunoprecipitation and direct competitive binding interaction through electrophoretic mobility shift assay. Acting as a regulatory docking platform, the long non-coding RNA PARTICLE serves to interlink epigenetic modification machineries and represents a compelling innovative component necessary for gene silencing on a global scale.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Histonas
/
Metilação de DNA
/
RNA Longo não Codificante
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article