Diverse Requirements for Microglial Survival, Specification, and Function Revealed by Defined-Medium Cultures.
Neuron
; 94(4): 759-773.e8, 2017 May 17.
Article
em En
| MEDLINE
| ID: mdl-28521131
ABSTRACT
Microglia, the resident macrophages of the CNS, engage in various CNS-specific functions that are critical for development and health. To better study microglia and the properties that distinguish them from other tissue macrophage populations, we have optimized serum-free culture conditions to permit robust survival of highly ramified adult microglia under defined-medium conditions. We find that astrocyte-derived factors prevent microglial death ex vivo and that this activity results from three primary components, CSF-1/IL-34, TGF-ß2, and cholesterol. Using microglial cultures that have never been exposed to serum, we demonstrate a dramatic and lasting change in phagocytic capacity after serum exposure. Finally, we find that mature microglia rapidly lose signature gene expression after isolation, and that this loss can be reversed by engrafting cells back into an intact CNS environment. These data indicate that the specialized gene expression profile of mature microglia requires continuous instructive signaling from the intact CNS.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fagocitose
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Sobrevivência Celular
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Colesterol
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Fator Estimulador de Colônias de Macrófagos
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Interleucinas
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Microglia
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Fator de Crescimento Transformador beta2
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article