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Structure and Intermolecular Interactions between L-Type Straight Flagellar Filaments.
Louzon, Daniel; Ginsburg, Avi; Schwenger, Walter; Dvir, Tom; Dogic, Zvonimir; Raviv, Uri.
Afiliação
  • Louzon D; The Institute of Chemistry and the Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem, Israel; The Racah Institute of Physics, The Hebrew University of Jerusalem, Jerusalem, Israel.
  • Ginsburg A; The Institute of Chemistry and the Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem, Israel; The Institute for Drug Research, The Hebrew University of Jerusalem, Jerusalem, Israel.
  • Schwenger W; Department of Physics, Brandeis University, Waltham, Massachusetts.
  • Dvir T; The Institute of Chemistry and the Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem, Israel; The Racah Institute of Physics, The Hebrew University of Jerusalem, Jerusalem, Israel.
  • Dogic Z; Department of Physics, Brandeis University, Waltham, Massachusetts.
  • Raviv U; The Institute of Chemistry and the Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem, Israel. Electronic address: uri.raviv@mail.huji.ac.il.
Biophys J ; 112(10): 2184-2195, 2017 May 23.
Article em En | MEDLINE | ID: mdl-28538155
Bacterial mobility is powered by rotation of helical flagellar filaments driven by rotary motors. Flagellin isolated from the Salmonella Typhimurium SJW1660 strain, which differs by a point mutation from the wild-type strain, assembles into straight filaments in which flagellin monomers are arranged in a left-handed helix. Using small-angle x-ray scattering and osmotic stress methods, we investigated the structure of SJW1660 flagellar filaments as well as the intermolecular forces that govern their assembly into dense hexagonal bundles. The scattering data were fitted to models, which took into account the atomic structure of the flagellin subunits. The analysis revealed the exact helical arrangement and the super-helical twist of the flagellin subunits within the filaments. Under osmotic stress, the filaments formed two-dimensional hexagonal bundles. Monte Carlo simulations and continuum theories were used to analyze the scattering data from hexagonal arrays, revealing how the bundle bulk modulus and the deflection length of filaments in the bundles depend on the applied osmotic stress. Scattering data from aligned flagellar bundles confirmed the theoretically predicated structure-factor scattering peak line shape. Quantitative analysis of the measured equation of state of the bundles revealed the contributions of electrostatic, hydration, and elastic interactions to the intermolecular forces associated with bundling of straight semi-flexible flagellar filaments.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Flagelos Tipo de estudo: Health_economic_evaluation / Prognostic_studies Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Flagelos Tipo de estudo: Health_economic_evaluation / Prognostic_studies Idioma: En Ano de publicação: 2017 Tipo de documento: Article