Selective fluorescence detection method for selenide and selenol using monochlorobimane.
Anal Biochem
; 532: 1-8, 2017 09 01.
Article
em En
| MEDLINE
| ID: mdl-28552757
ABSTRACT
The low redox potential of selenide and selenol is physiologically important, as it confers efficient catalytic abilities to selenoproteins. Quantitative determination of selenol and selenide provide important clues for understanding the metabolism and physiological function of selenium. However, selective detection of selenol and selenide is extremely difficult because of their chemical similarity to thiol and sulfide. In this study, we established a highly sensitive, selective, quantitative, and simple method for detection of selenol and selenide, using a reaction with monochlorobimane (MCB), followed by ethyl acetate extraction of the product syn-(methyl,methyl)bimane. We analyzed selenide production from selenite, catalyzed by human glutathione reductase, and also determined selenide and selenol concentrations in Hepa1-6 cells using the MCB method, to demonstrate its practical applications. This study provides a new tool for selenium detection in biology.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Pirazóis
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Selenito de Sódio
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Compostos de Selênio
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Carcinoma Hepatocelular
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Fluorescência
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Neoplasias Hepáticas
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article