In vitro evaluation of gel-encapsulated adipose derived stem cells: Biochemical cues for in vivo peripheral nerve repair.
J Tissue Eng Regen Med
; 12(3): 676-686, 2018 03.
Article
em En
| MEDLINE
| ID: mdl-28556533
ABSTRACT
Adipose-derived stem cells (ASC) are becoming one of the most exploited cells in peripheral nerve repair. They are fast-growing and able to protect neurons from apoptosis; they can reduce post-injury latency and the risk of muscle atrophy. This study evaluates laminin-loaded fibrin gel as an ASC-carrying scaffold for nerve repair. In vitro, ASC retained their proliferative activity but showed significant increase in proliferation rate when encapsulated in gels with low laminin concentrations (i.e., 1 µg/mL). We observed a linear decrease of ASC proliferation rate with increasing laminin concentration from 1 to 100 µg/mL. We next examined the effect of the ASC-carrying fibrin gels on in vitro dorsal root ganglia (DRG) neurite extension, then in vivo sciatic nerve regeneration in adult rats. The ASC-carrying gel was embedded in 15-mm-long, 1.5-mm-diameter polydimethylsiloxane regenerative conduits for in vivo evaluation. At 8-week post implantation, robust regeneration was observed across the long gap. Taken together, these results suggest ASC-carrying gels are a potential path to improve the efficacy of nerve regeneration through artificial guidance conduits and electrode nerve interfaces.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Nervos Periféricos
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Tecido Adiposo
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Células Imobilizadas
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Células-Tronco Mesenquimais
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Géis
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Regeneração Nervosa
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article