miR-100 promotes the proliferation of spermatogonial stem cells via regulating Stat3.
Mol Reprod Dev
; 84(8): 693-701, 2017 Aug.
Article
em En
| MEDLINE
| ID: mdl-28569396
ABSTRACT
Micro RNAs play important roles during mammalian spermatogenesis, but the function(s) of specific miRNAs remain largely unknown. Here, we report that miR-100 is predominantly expressed in undifferentiated murine spermatogonia, including spermatogonial stem cells (SSCs). We utilized a miRNA mimic and inhibitor to knock down and overexpress miR-100 in cultured SSCs, respectively, finding that the miR-100 promotes the proliferation of SSCs in vitro. Furthermore, signals promoting SSC maintenance induced, whereas retinoic acid repressed, expression of miR-100. Stat3 expression was modulated by miR-100, with increased transcript and protein abundance in the presence of the miR-100 inhibitor versus reduced protein levels following miR-100 overexpression. Stat3 silencing also mimicked the reduced SSC proliferation phenotype associated with elevated miR-100 levels. Importantly, Stat3 silencing rescued the anti-proliferation capacity of miR-100 inhibitor on cultured SSCs. Given that the Stat3 3' untranslated region was not repressed by pre-miR-100 in a standard luciferase reporter assay, we suggest that miR-100 promotes SSC proliferation by indirect regulation of Stat3.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Espermatogênese
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Transdução de Sinais
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MicroRNAs
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Proliferação de Células
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Fator de Transcrição STAT3
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article