Diverse stimuli engage different neutrophil extracellular trap pathways.
Elife
; 62017 06 02.
Article
em En
| MEDLINE
| ID: mdl-28574339
ABSTRACT
Neutrophils release neutrophil extracellular traps (NETs) which ensnare pathogens and have pathogenic functions in diverse diseases. We examined the NETosis pathways induced by five stimuli; PMA, the calcium ionophore A23187, nigericin, Candida albicans and Group B Streptococcus. We studied NET production in neutrophils from healthy donors with inhibitors of molecules crucial to PMA-induced NETs including protein kinase C, calcium, reactive oxygen species, the enzymes myeloperoxidase (MPO) and neutrophil elastase. Additionally, neutrophils from chronic granulomatous disease patients, carrying mutations in the NADPH oxidase complex or a MPO-deficient patient were examined. We show that PMA, C. albicans and GBS use a related pathway for NET induction, whereas ionophores require an alternative pathway but that NETs produced by all stimuli are proteolytically active, kill bacteria and composed mainly of chromosomal DNA. Thus, we demonstrate that NETosis occurs through several signalling mechanisms, suggesting that extrusion of NETs is important in host defence.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Armadilhas Extracelulares
/
Neutrófilos
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article