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Transfection of Antisense Oligonucleotides Mediated by Cationic Vesicles Based on Non-Ionic Surfactant and Polycations Bearing Quaternary Ammonium Moieties.
Mayr, Judith; Grijalvo, Santiago; Bachl, Jürgen; Pons, Ramon; Eritja, Ramon; Díaz Díaz, David.
Afiliação
  • Mayr J; Institut für Organische Chemie, Universität Regensburg, Universitätsstr. 31, Regensburg 93053, Germany. judith.mayr@chemie.uni-regensburg.de.
  • Grijalvo S; Institute of Advanced Chemistry of Catalonia-Spanish National Research Council (IQAC-CSIC), Jordi Girona 18-26, Barcelona 08034, Spain. sgrgma@cid.csic.es.
  • Bachl J; Networking Centre in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Jordi Girona 18-26, Barcelona 08034, Spain. sgrgma@cid.csic.es.
  • Pons R; Institut für Organische Chemie, Universität Regensburg, Universitätsstr. 31, Regensburg 93053, Germany. bachl_j@web.de.
  • Eritja R; Institute of Advanced Chemistry of Catalonia-Spanish National Research Council (IQAC-CSIC), Jordi Girona 18-26, Barcelona 08034, Spain. ramon.pons@iqac.csic.es.
  • Díaz Díaz D; Institute of Advanced Chemistry of Catalonia-Spanish National Research Council (IQAC-CSIC), Jordi Girona 18-26, Barcelona 08034, Spain. ramon.eritja@iqac.csic.es.
Int J Mol Sci ; 18(6)2017 May 26.
Article em En | MEDLINE | ID: mdl-28587106
Three different ionene polymers with varying quaternary ammonium moieties were used as a proof of concept for the formulation of antisense oligonucleotides, which are capable of inhibiting Renilla luciferase messenger ribonucleic acid (mRNA). Cationic vesicles, consisting of cationic polymer, antisense oligonucleotide (Luc) and non-ionic surfactant polysorbate 80, were investigated regarding their ζ potential, cytotoxicity and transfection efficiency. Deoxyribonucleic acid- (DNA) forming complexes in the presence of cationic vesicles were also investigated in terms of small-angle X-ray scattering (SAXS). The studied cationic vesicles showed very little, if any, toxicity against HeLa cells. Transfection abilities proved to vary strongly depending on the present quaternary ammonium moiety.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Poliaminas / Polímeros / Tensoativos / Transfecção / Oligonucleotídeos Antissenso / Compostos de Amônio Quaternário Limite: Humans Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Poliaminas / Polímeros / Tensoativos / Transfecção / Oligonucleotídeos Antissenso / Compostos de Amônio Quaternário Limite: Humans Idioma: En Ano de publicação: 2017 Tipo de documento: Article