[Expression of ß-integrin family members in children with T-cell acute lymphoblastic leukemia].
Zhongguo Dang Dai Er Ke Za Zhi
; 19(6): 620-626, 2017 Jun.
Article
em Zh
| MEDLINE
| ID: mdl-28606226
ABSTRACT
OBJECTIVE:
To study the expression of ß-integrin family members in children with T-cell acute lymphoblastic leukemia (T-ALL) and their significance.METHODS:
Quantitative real-time PCR analyses were performed to assess the expression levels of ß-integrin family members in bone marrow samples from 22 children with newly-diagnosed T-ALL and 21 controls (16 children with non-malignant hematologic disease and 5 healthy donors with bone marrow transplantation). Jurkat cells were treated with integrin inhibitor arginine-glycine-aspartate (Arg-Gly-Asp, RGD) peptide. The cell viability and apoptosis rate were determined by CCK8 assay and flow cytometry respectively.RESULTS:
The mRNA levels of integrins ß2, ß3, and ß5 were significantly lower in children with T-ALL than in controls (P<0.05). In T-ALL patients, high integrin ß3 expression was associated with lower white blood cell counts (<100×109/L), minimal residual disease (MRD) positivity, and day 33 bone marrow negative remission (P<0.05). In T-ALL patients, higher integrin ß5 expression was associated with relapse of T-ALL (P<0.05). Based on survival curve analysis, higher integrin ß3 expression was related to lower event-free survival and overall survival rates. RGD peptide treatment inhibited the proliferation of Jurkat cells and increased their apoptosis rate (P<0.05).CONCLUSIONS:
ß-Integrin may play a role in the occurrence and development of T-ALL by affecting cell proliferation and apoptosis. The expression of integrin ß5 is closely related to the risk of relapse of T-ALL. The expression of integrin ß3 is closely related the treatment response and prognosis of T-ALL.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cadeias beta de Integrinas
/
Leucemia-Linfoma Linfoblástico de Células T Precursoras
Tipo de estudo:
Etiology_studies
Limite:
Child
/
Child, preschool
/
Female
/
Humans
/
Male
Idioma:
Zh
Ano de publicação:
2017
Tipo de documento:
Article