In Saccharomyces cerevisiae gene targeting fidelity depends on a transformation method and proportion of the overall length of the transforming and targeted DNA.

Stafa, Anamarija; Miklenic, Marina Svetec; Zandona, Antonio; Zunar, Bojan; Cadez, Neza; Petkovic, Hrvoje; Svetec, Ivan Kresimir

Stafa, Anamarija; Miklenic, Marina Svetec; Zandona, Antonio; Zunar, Bojan; Cadez, Neza; Petkovic, Hrvoje; Svetec, Ivan Kresimir.

Afiliação

Stafa A; Laboratory for Biology and Microbial Genetics, Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Krsnjavoga 25, 10000 Zagreb, Croatia.
Miklenic MS; Laboratory for Biology and Microbial Genetics, Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Krsnjavoga 25, 10000 Zagreb, Croatia.
Zandona A; Laboratory for Biology and Microbial Genetics, Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Krsnjavoga 25, 10000 Zagreb, Croatia.
Zunar B; Laboratory for Biology and Microbial Genetics, Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Krsnjavoga 25, 10000 Zagreb, Croatia.
Cadez N; Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia.
Petkovic H; Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia.
Svetec IK; Laboratory for Biology and Microbial Genetics, Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Krsnjavoga 25, 10000 Zagreb, Croatia.

FEMS Yeast Res ; 17(4)2017 06 01.

Article em En | MEDLINE | ID: mdl-28633406

Assuntos

DNA/genética; Marcação de Genes/métodos; Genoma Fúngico; Plasmídeos/química; Saccharomyces cerevisiae/genética; Transfecção/métodos; Acetatos/química; Sequência de Bases; Duplicação Cromossômica; DNA/metabolismo; Eletroporação; Cariotipagem; Plasmídeos/metabolismo; Ploidias; Saccharomyces cerevisiae/metabolismo; Esferoplastos/genética; Esferoplastos/metabolismo; Transformação Genética

Palavras-chave

aneuploidy; diploidisation; gene targeting fidelity; off-targeting events; targeted chromosome duplication; yeast transformation

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Plasmídeos / Saccharomyces cerevisiae / DNA / Transfecção / Genoma Fúngico / Marcação de Genes Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google