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Lack of a peroxiredoxin suppresses the lethality of cells devoid of electron donors by channelling electrons to oxidized ribonucleotide reductase.
Boronat, Susanna; Domènech, Alba; Carmona, Mercè; García-Santamarina, Sarela; Bañó, M Carmen; Ayté, José; Hidalgo, Elena.
Afiliação
  • Boronat S; Oxidative Stress and Cell Cycle Group, Universitat Pompeu Fabra, Barcelona, Spain.
  • Domènech A; Oxidative Stress and Cell Cycle Group, Universitat Pompeu Fabra, Barcelona, Spain.
  • Carmona M; Oxidative Stress and Cell Cycle Group, Universitat Pompeu Fabra, Barcelona, Spain.
  • García-Santamarina S; Oxidative Stress and Cell Cycle Group, Universitat Pompeu Fabra, Barcelona, Spain.
  • Bañó MC; Departamento de Bioquímica y Biología Molecular, Universitat de València, Valencia, Spain.
  • Ayté J; Oxidative Stress and Cell Cycle Group, Universitat Pompeu Fabra, Barcelona, Spain.
  • Hidalgo E; Oxidative Stress and Cell Cycle Group, Universitat Pompeu Fabra, Barcelona, Spain.
PLoS Genet ; 13(6): e1006858, 2017 Jun.
Article em En | MEDLINE | ID: mdl-28640807
ABSTRACT
The thioredoxin and glutaredoxin pathways are responsible of recycling several enzymes which undergo intramolecular disulfide bond formation as part of their catalytic cycles such as the peroxide scavengers peroxiredoxins or the enzyme ribonucleotide reductase (RNR). RNR, the rate-limiting enzyme of deoxyribonucleotide synthesis, is an essential enzyme relying on these electron flow cascades for recycling. RNR is tightly regulated in a cell cycle-dependent manner at different levels, but little is known about the participation of electron donors in such regulation. Here, we show that cytosolic thioredoxins Trx1 and Trx3 are the primary electron donors for RNR in fission yeast. Unexpectedly, trx1 transcript and Trx1 protein levels are up-regulated in a G1-to-S phase-dependent manner, indicating that the supply of electron donors is also cell cycle-regulated. Indeed, genetic depletion of thioredoxins triggers a DNA replication checkpoint ruled by Rad3 and Cds1, with the final goal of up-regulating transcription of S phase genes and constitutive RNR synthesis. Regarding the thioredoxin and glutaredoxin cascades, one combination of gene deletions is synthetic lethal in fission yeast cells lacking both thioredoxin reductase and cytosolic dithiol glutaredoxin. We have isolated a suppressor of this lethal phenotype a mutation at the Tpx1-coding gene, leading to a frame shift and a loss-of-function of Tpx1, the main client of electron donors. We propose that in a mutant strain compromised in reducing equivalents, the absence of an abundant and competitive substrate such as the peroxiredoxin Tpx1 has been selected as a lethality suppressor to favor RNR function at the expense of the non-essential peroxide scavenging function, to allow DNA synthesis and cell growth.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ribonucleotídeo Redutases / Tiorredoxinas / Proteínas de Schizosaccharomyces pombe / Transporte de Elétrons / Peroxirredoxinas Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ribonucleotídeo Redutases / Tiorredoxinas / Proteínas de Schizosaccharomyces pombe / Transporte de Elétrons / Peroxirredoxinas Idioma: En Ano de publicação: 2017 Tipo de documento: Article