Expression of granulocyte colony-stimulating factor 3 receptor in the spinal dorsal horn following spinal nerve ligation-induced neuropathic pain.
Mol Med Rep
; 16(2): 2009-2015, 2017 Aug.
Article
em En
| MEDLINE
| ID: mdl-28656207
ABSTRACT
In previous studies that have profiled gene expression in patients with complex regional pain syndrome (CRPS), the expression of granulocyte colony-stimulating factor 3 receptor (GCSFR) was elevated, as were a number of painassociated genes. The present study determined the expression of GCSFR and the mechanisms by which it may affect hypersensitivity, focusing on the signal transducer and activator of transcription 3 (STAT3)/transient receptor potential cation channel subfamily V 1 (TRPV1) signaling pathway in particular, which is an important mediator of pain. Following L5 spinal nerve ligation (SNL) surgery, the protein and mRNA levels of GCSFR increased in the ipsilateral spinal dorsal horn when compared with the sham and/or contralateral control. Double immunofluorescence further demonstrated that GCSFR colocalized with TRPV1 and phosphorylated STAT in the neurons of the spinal dorsal horn. GCSF treatment led to an increase in GCSFR and TRPV1 expression and phosphorylation of STAT3. These results indicate that GCSFinduced GCSFR expression may activate TRPV1 by promoting phosphorylation of STAT3. Collectively, the results suggest, for the first time, that the expression of GCSFR in neurons following peripheral nerve injury may be involved in the induction and maintenance of neuropathic pain through the STAT3 and TRPV1 signaling pathway.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Nervos Espinhais
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Receptores de Fator Estimulador de Colônias
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Corno Dorsal da Medula Espinal
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Neuralgia
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article