An improved PCR-CTPP assay for the detection of ADH1B Arg48His polymorphism.
J Clin Lab Anal
; 32(2)2018 Feb.
Article
em En
| MEDLINE
| ID: mdl-28657176
ABSTRACT
BACKGROUND:
ADH1B Arg48His polymorphism is associated with the development of alcohol-related diseases. In this study, we aimed to explore an improved polymerase chain reaction with confronting two-pair primers (PCR-CTPP) assay for the detection of ADH1B Arg48His polymorphism.METHODS:
A mismatch was introduced at the 3' end of each of the two allele-specific to increase the specificity of the reaction. But beyond that, a new mismatch at-3 positions of outer primers was designed to decrease the efficiency of the aforementioned primers and depresses the amplification of an internal nonspecific DNA control. A total of 180 samples from healthy volunteers Han Chinese were tested to evaluate this new assay.RESULTS:
The protocol of PCR-CTPP was successful for genotyping of ADH1B Arg48His. The results from the improved PCR-CTPP assay were confirmed by Sanger sequencing, and correct genotyping rates were 100%.The genotype frequencies were 49.44% (89 cases) for His/His, 46.67% (84 cases) for Arg/His, and 3.89% (seven cases) for Arg/Arg respectively.CONCLUSIONS:
This improved PCR-CTPP assay is simple, rapid, cost-effective, and reliable, specific for the detection of ADH1B Arg48His polymorphism in most clinical diagnostic laboratories.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Álcool Desidrogenase
/
Reação em Cadeia da Polimerase
/
Análise de Sequência de DNA
/
Polimorfismo de Nucleotídeo Único
Tipo de estudo:
Diagnostic_studies
/
Guideline
Limite:
Female
/
Humans
/
Male
País como assunto:
Asia
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article