Isolation of Premyrsinane, Myrsinane, and Tigliane Diterpenoids from Euphorbia pithyusa Using a Chikungunya Virus Cell-Based Assay and Analogue Annotation by Molecular Networking.
J Nat Prod
; 80(7): 2051-2059, 2017 07 28.
Article
em En
| MEDLINE
| ID: mdl-28671832
Six new premyrsinol esters (1-6) and one new myrsinol ester (8) were isolated from an aerial parts extract of Euphorbia pithyusa, together with a known premyrsinol (7) and two known dideoxyphorbol esters (9 and 10), following a bioactivity-guided purification procedure using a chikungunya virus (CHIKV) cell-based assay. The structures of the new diterpene esters (1-6 and 8) were elucidated by MS and NMR spectroscopic data interpretation. Compounds 1-10 were evaluated against CHIKV replication, and results showed that the 4ß-dideoxyphorbol ester 10 was the most active compound, with an EC50 value of 4.0 ± 0.3 µM and a selectivity index of 10.6. To gain more insight into the structural diversity of diterpenoids produced by E. pithyusa, the initial extract and chromatographic fractions were analyzed by LC-MS/MS. The generated data were annotated using a molecular networking procedure and revealed that dozens of unknown premyrsinane, myrsinane, and tigliane analogues were present.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Antivirais
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Vírus Chikungunya
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Euphorbia
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Diterpenos
Limite:
Animals
País como assunto:
Europa
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article