AuNP-CTG based probing system targeting CAG repeat DNA and RNA sequences.
Bioorg Med Chem Lett
; 27(16): 3772-3775, 2017 08 15.
Article
em En
| MEDLINE
| ID: mdl-28687202
ABSTRACT
We have developed a AuNP-CTG based probing system that is applicable to the detection of many units of CAG repeat sequences which was synthesized by a rolling circle amplification (RCA) system with changes in fluorescence. We also demonstrate that our AuNP-CTG based probing system could transfect without using transfection reagent and detect target CAG repeat sequences in HeLa cells with dramatic changes in fluorescence. This AuNP-CTG based probing system could also be used, in conjunction with the CAG repeat RCA system, to detect target DNA. This system was so sensitive to the target DNA that it could detect even picomolar amounts with amplification of the fluorescence signal. Furthermore, we have used our gold-based CAG probing system for the detection of RNA CAG repeat sequences.
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Base de dados:
MEDLINE
Assunto principal:
DNA
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RNA
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Sondas de DNA
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Nanopartículas
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Ouro
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article