Immunohistochemical localization of histidine-rich glycoprotein in human skeletal muscle: preferential distribution of the protein at the sarcomeric I-band.
Histochem Cell Biol
; 148(6): 651-657, 2017 Dec.
Article
em En
| MEDLINE
| ID: mdl-28702782
ABSTRACT
Histidine-rich glycoprotein (HRG) is a relatively abundant plasma protein that is synthesized by parenchymal liver cells. Using Western blot analysis and immunoperoxidase techniques, we have previously shown the presence of HRG in human skeletal muscle. This paper reports the results of immunofluorescence experiments carried out on sections of human normal skeletal muscle biopsies to investigate the subcellular localization of HRG. The HRG localization was also compared with that of skeletal muscle AMP deaminase (AMPD1), since we have previously described an association of the enzyme with the protein. The obtained results give evidence for a preferential localization of HRG at the I-band level, where it shows the same distribution of actin and where AMPD1 is present in major concentration.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Sarcômeros
/
Proteínas
/
Músculo Esquelético
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article