Detection of rotavirus in clinical specimens using an immunosensor prototype based on the photon burst counting technique.
Biomed Opt Express
; 8(7): 3383-3394, 2017 Jul 01.
Article
em En
| MEDLINE
| ID: mdl-28717574
ABSTRACT
In this study, a sensitive fluorescence sensor was developed for the detection of small, fluorescence-labeled particles dispersed in a solution. The prototype system comprises of a laser confocal optical system and a mechanical sample stage to detect photon bursting of fluorescence-labeled small particles in sample volumes less than 5 µL within 3 minutes. To examine the feasibility of the prototype system as a diagnostic tool, assemblages of rotavirus and fluorescence-labeled antibody were analyzed. The detection sensitivity for rotavirus was 1 × 104 pfu/mL. Rotavirus in stool samples from patients with acute gastroenteritis was also detected. The advantages and disadvantages of this immunosensor with respect to ELISA and RT-PCR, the current gold standards for virus detection, are discussed.
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Base de dados:
MEDLINE
Tipo de estudo:
Diagnostic_studies
/
Guideline
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article