[Results of thalassemia screening and genetic diagnosis for 13 738 pregnant women].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
; 34(4): 588-591, 2017 Aug 10.
Article
em Zh
| MEDLINE
| ID: mdl-28777865
ABSTRACT
OBJECTIVE:
To report on the result of thalassemia screening and genetic diagnosis for pregnant women from Guiyang region.METHODS:
Prenatal screening for thalassemia was carried out based on erythrocyte parameters and hemoglobin electrophoresis. Single-tube multiplex GAP-PCR and PCR-reverse dot blot hybridization were performed on suspected cases to identify common alpha- and beta- thalassemia mutations, and direct sequencing was used for identifying rare mutations.RESULTS:
Among 13 738 pregnant women, 1745 (12.70%) were suspected as thalassemia. In terms of native place, the provinces with highest screening-positive rates were Guangxi, Guangdong, Jiangxi and Guizhou. And the ethnic groups with highest screening-positive rates were Zhuang, Li, and Buyi. Among 801 women subjected to genetic testing, 457 (57.05%) were diagnosed with thalassemia. In total 9 genotypes of alpha- thalassemia were detected, with the most common genotypes being --SEA/alpha alpha (63.35%), - alpha3.7/alpha alpha (19.37%) and - alpha4.2/alpha alpha (8.90%). Eleven genotypes of beta- thalassemia were detected, with the most common genotypes being CD17/N (42.91%), CD41-42/N (32.46%) and IVS-II-654/N (11.94%). Two cases were detected with rare beta-thalassemia mutations (CD54-58/N and IVS-I-130/N).CONCLUSION:
The screening-positive rate of thalassemia among pregnant women in Guiyang region is relatively high. The rates have shown substantial difference in terms of native place and ethnic group. Thalassemia-related mutations in Guizhou region have a diverse spectrum, which showed certain difference from those of other regions.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Talassemia
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Adolescent
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Adult
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Female
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Humans
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Middle aged
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Pregnancy
Idioma:
Zh
Ano de publicação:
2017
Tipo de documento:
Article