Your browser doesn't support javascript.
loading
From Macrocrystals to Microcrystals: A Strategy for Membrane Protein Serial Crystallography.
Dods, Robert; Båth, Petra; Arnlund, David; Beyerlein, Kenneth R; Nelson, Garrett; Liang, Mengling; Harimoorthy, Rajiv; Berntsen, Peter; Malmerberg, Erik; Johansson, Linda; Andersson, Rebecka; Bosman, Robert; Carbajo, Sergio; Claesson, Elin; Conrad, Chelsie E; Dahl, Peter; Hammarin, Greger; Hunter, Mark S; Li, Chufeng; Lisova, Stella; Milathianaki, Despina; Robinson, Joseph; Safari, Cecilia; Sharma, Amit; Williams, Garth; Wickstrand, Cecilia; Yefanov, Oleksandr; Davidsson, Jan; DePonte, Daniel P; Barty, Anton; Brändén, Gisela; Neutze, Richard.
Afiliação
  • Dods R; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
  • Båth P; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
  • Arnlund D; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
  • Beyerlein KR; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Hamburg, Germany.
  • Nelson G; Department of Physics, Arizona State University, Tempe, AZ, USA.
  • Liang M; Linac Coherent Light Source, Stanford Linear Accelerator Center (SLAC) National Accelerator Laboratory, Menlo Park, CA, USA.
  • Harimoorthy R; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
  • Berntsen P; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden; Australian Research Council Centre of Excellence in Advanced Molecular Imaging, La Trobe Institute for Molecular Science, La Trobe University, Melbourne 3086, Australia.
  • Malmerberg E; Physical Biosciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Rd, Berkeley, CA 94720, USA.
  • Johansson L; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden; The Bridge Institute, Department of Chemistry, University of Southern California, Los Angeles, CA 90089-3303, USA.
  • Andersson R; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
  • Bosman R; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
  • Carbajo S; Linac Coherent Light Source, Stanford Linear Accelerator Center (SLAC) National Accelerator Laboratory, Menlo Park, CA, USA.
  • Claesson E; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
  • Conrad CE; Department of Physics, Arizona State University, Tempe, AZ, USA.
  • Dahl P; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
  • Hammarin G; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
  • Hunter MS; Linac Coherent Light Source, Stanford Linear Accelerator Center (SLAC) National Accelerator Laboratory, Menlo Park, CA, USA.
  • Li C; Department of Physics, Arizona State University, Tempe, AZ, USA.
  • Lisova S; Department of Physics, Arizona State University, Tempe, AZ, USA.
  • Milathianaki D; Linac Coherent Light Source, Stanford Linear Accelerator Center (SLAC) National Accelerator Laboratory, Menlo Park, CA, USA.
  • Robinson J; Linac Coherent Light Source, Stanford Linear Accelerator Center (SLAC) National Accelerator Laboratory, Menlo Park, CA, USA.
  • Safari C; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
  • Sharma A; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
  • Williams G; Linac Coherent Light Source, Stanford Linear Accelerator Center (SLAC) National Accelerator Laboratory, Menlo Park, CA, USA.
  • Wickstrand C; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
  • Yefanov O; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Hamburg, Germany.
  • Davidsson J; Department of Photochemistry and Molecular Science, Uppsala University, Uppsala, Sweden.
  • DePonte DP; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Hamburg, Germany.
  • Barty A; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Hamburg, Germany.
  • Brändén G; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden. Electronic address: gisela.branden@gu.se.
  • Neutze R; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden. Electronic address: richard.neutze@gu.se.
Structure ; 25(9): 1461-1468.e2, 2017 09 05.
Article em En | MEDLINE | ID: mdl-28781082
ABSTRACT
Serial protein crystallography was developed at X-ray free-electron lasers (XFELs) and is now also being applied at storage ring facilities. Robust strategies for the growth and optimization of microcrystals are needed to advance the field. Here we illustrate a generic strategy for recovering high-density homogeneous samples of microcrystals starting from conditions known to yield large (macro) crystals of the photosynthetic reaction center of Blastochloris viridis (RCvir). We first crushed these crystals prior to multiple rounds of microseeding. Each cycle of microseeding facilitated improvements in the RCvir serial femtosecond crystallography (SFX) structure from 3.3-Å to 2.4-Å resolution. This approach may allow known crystallization conditions for other proteins to be adapted to exploit novel scientific opportunities created by serial crystallography.
Assuntos
Palavras-chave
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Hyphomicrobiaceae / Proteínas de Membrana Idioma: En Ano de publicação: 2017 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Hyphomicrobiaceae / Proteínas de Membrana Idioma: En Ano de publicação: 2017 Tipo de documento: Article