Human Centromeres Produce Chromosome-Specific and Array-Specific Alpha Satellite Transcripts that Are Complexed with CENP-A and CENP-C.
Dev Cell
; 42(3): 226-240.e6, 2017 08 07.
Article
em En
| MEDLINE
| ID: mdl-28787590
ABSTRACT
Human centromeres are defined by alpha satellite DNA arrays that are distinct and chromosome specific. Most human chromosomes contain multiple alpha satellite arrays that are competent for centromere assembly. Here, we show that human centromeres are defined by chromosome-specific RNAs linked to underlying organization of distinct alpha satellite arrays. Active and inactive arrays on the same chromosome produce discrete sets of transcripts in cis. Non-coding RNAs produced from active arrays are complexed with CENP-A and CENP-C, while inactive-array transcripts associate with CENP-B and are generally less stable. Loss of CENP-A does not affect transcript abundance or stability. However, depletion of array-specific RNAs reduces CENP-A and CENP-C at the targeted centromere via faulty CENP-A loading, arresting cells before mitosis. This work shows that each human alpha satellite array produces a unique set of non-coding transcripts, and RNAs present at active centromeres are necessary for kinetochore assembly and cell-cycle progression.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Autoantígenos
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DNA Satélite
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Proteínas Cromossômicas não Histona
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Centrômero
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Cromossomos
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RNA não Traduzido
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article