Degree of Biomimicry of Artificial Spider Silk Spinning Assessed by NMR Spectroscopy.
Angew Chem Int Ed Engl
; 56(41): 12571-12575, 2017 10 02.
Article
em En
| MEDLINE
| ID: mdl-28791761
ABSTRACT
Biomimetic spinning of artificial spider silk requires that the terminal domains of designed minispidroins undergo specific structural changes in concert with the ß-sheet conversion of the repetitive region. Herein, we combine solution and solid-state NMR methods to probe domain-specific structural changes in the NT2RepCT minispidroin, which allows us to assess the degree of biomimicry of artificial silk spinning. In addition, we show that the structural effects of post-spinning procedures can be examined. By studying the impact of NT2RepCT fiber drying, we observed a reversible beta-to-alpha conversion. We think that this approach will be useful for guiding the optimization of artificial spider silk fibers.
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Base de dados:
MEDLINE
Assunto principal:
Aranhas
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Materiais Biomiméticos
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Seda
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Proteínas de Artrópodes
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article