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3M-052 as an adjuvant for a PLGA microparticle-based Leishmania donovani recombinant protein vaccine.
Wang, Qian; Barry, Meagan A; Seid, Christopher A; Hudspeth, Elissa M; McAtee, C Patrick; Heffernan, Michael J.
Afiliação
  • Wang Q; Department of Pediatrics (Section of Tropical Medicine), National School of Tropical Medicine, Baylor College of Medicine, Houston, Texas.
  • Barry MA; Texas Children's Hospital Center for Vaccine Development, Houston, Texas.
  • Seid CA; Department of Pediatrics (Section of Tropical Medicine), National School of Tropical Medicine, Baylor College of Medicine, Houston, Texas.
  • Hudspeth EM; Texas Children's Hospital Center for Vaccine Development, Houston, Texas.
  • McAtee CP; Department of Pediatrics (Section of Tropical Medicine), National School of Tropical Medicine, Baylor College of Medicine, Houston, Texas.
  • Heffernan MJ; Texas Children's Hospital Center for Vaccine Development, Houston, Texas.
J Biomed Mater Res B Appl Biomater ; 106(4): 1587-1594, 2018 05.
Article em En | MEDLINE | ID: mdl-28804955
It is believed that an effective vaccine against leishmaniasis will require a T helper type 1 (TH 1) immune response. In this study, we investigated the adjuvanticity of the Toll-like receptor (TLR) 7/8 agonist 3M-052 in combination with the Leishmania donovani 36-kDa nucleoside hydrolase recombinant protein antigen (NH36). NH36 and 3M-052 were encapsulated in separate batches of poly(lactic-co-glycolic acid) (PLGA) microparticles (MPs). The loading efficiency for NH36 was 83% and for 3M-052 was above 95%. In vitro stimulation of bone marrow-derived dendritic cells, measured by IL-12 secretion, demonstrated that 3M-052 (free or MP-formulated) had a concentration-dependent immunostimulatory effect with an optimum concentration of 2 µg/mL. In immunogenicity studies in BALB/c mice, MP-formulated NH36 and 3M-052 elicited the highest serum titers of TH 1-associated IgG2a and IgG2b antibodies and the highest frequency of IFNγ-producing splenocytes. No dose dependency was observed among MP/NH36/3M-052 groups over a dose range of 4-60 µg 3M-052 per injection. The ability of MP-formulated NH36 and 3M-052 to elicit a TH 1-biased immune response indicates the potential for PLGA MP-formulated 3M-052 to be used as an adjuvant for leishmaniasis vaccines. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1587-1594, 2018.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ácidos Esteáricos / Leishmania donovani / Proteínas de Protozoários / Vacinas contra Leishmaniose / Copolímero de Ácido Poliláctico e Ácido Poliglicólico / Compostos Heterocíclicos com 3 Anéis / Leishmaniose Visceral / Antígenos de Protozoários Limite: Animals Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ácidos Esteáricos / Leishmania donovani / Proteínas de Protozoários / Vacinas contra Leishmaniose / Copolímero de Ácido Poliláctico e Ácido Poliglicólico / Compostos Heterocíclicos com 3 Anéis / Leishmaniose Visceral / Antígenos de Protozoários Limite: Animals Idioma: En Ano de publicação: 2018 Tipo de documento: Article