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Production of Single Contracting Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes: Matrigel Mattress Technique.
Cadar, Adrian G; Feaster, Tromondae K; Durbin, Matthew D; Hong, Charles C.
Afiliação
  • Cadar AG; Department of Molecular Physiology & Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee.
  • Feaster TK; Department of Medicine/Division of Cardiovascular Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee.
  • Durbin MD; Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennessee.
  • Hong CC; Department of Pediatrics/Division of Neonatology, Indiana University School of Medicine, Indianapolis, Indiana.
Curr Protoc Stem Cell Biol ; 42: 4A.14.1-4A.14.7, 2017 Aug 14.
Article em En | MEDLINE | ID: mdl-28806851
ABSTRACT
This unit describes the published Matrigel mattress method. Briefly, we describe the preparation of the mattress, replating of the human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) on the Matrigel mattress, and hiPSC-CM mattress maintenance. Adherence to this protocol will yield individual, robustly shortening hiPSC-CMs, which can be used for downstream applications. © 2017 by John Wiley & Sons, Inc.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteoglicanas / Colágeno / Laminina / Técnicas de Cultura de Células / Miócitos Cardíacos / Células-Tronco Pluripotentes Induzidas / Contração Miocárdica Limite: Humans Idioma: En Ano de publicação: 2017 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteoglicanas / Colágeno / Laminina / Técnicas de Cultura de Células / Miócitos Cardíacos / Células-Tronco Pluripotentes Induzidas / Contração Miocárdica Limite: Humans Idioma: En Ano de publicação: 2017 Tipo de documento: Article