Cumulibacter manganitolerans gen. nov., sp. nov., isolated from sludge of a manganese mine.
Int J Syst Evol Microbiol
; 67(8): 2646-2652, 2017 Aug.
Article
em En
| MEDLINE
| ID: mdl-28809149
ABSTRACT
A Gram-stain-positive actinobacterium, designated as strain 2-36T, was isolated from sludge of a sewage outlet in a manganese mine. Phylogenetic analysis of 16S rRNA genes assigned strain 2-36T in a single lineage in the family Geodermatophilaceae and closely related to Modestobacter roseus KLBMP 1279T (93.8â% similarity), Blastococcus saxobsidens BC444T (93.1â%) and Geodermatophilus aquaeductus BMG801T (92.8â%). It contained iso-C16ââ0, iso-C15ââ0, C17ââ1ω6c, iso-C14ââ0 and summed feature 3 (C16ââ1ω6c and/or C16ââ1ω7c) as the major fatty acids (>5â%), with MK-9(H4), MK-8(H4) and MK-9(H6) as the quinones. The cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid and the DNA G+C content was 70.1 mol%. The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, an unknown glycolipid, an unknown aminophospholipid and two unknown phospholipids. Compared to closely related strains, strain 2-36T showed distinguishing characteristics, such as the distinct phylogenetic lineage, positive result for phenylacetic acid assimilation and negative result for α-glucosidase and having C17ââ1ω6c and iso-C14ââ0 as the major fatty acids. On the basis of the polyphasic analyses, strain 2-36T represents a novel species of a new genus within the family Geodermatophilaceae, for which the name Cumulibacter manganitolerans gen. nov., sp. nov. is proposed. The type strain of 'Cumulibacter manganitolerans' is 2-36T (=CCTCC AA 2016026T=DSM 103787T).
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Filogenia
/
Esgotos
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Actinomycetales
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Mineração
País como assunto:
Asia
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article