Hybrid photoacoustic and electrophysiological recording of neurovascular communications in freely-moving rats.
Neuroimage
; 161: 232-240, 2017 11 01.
Article
em En
| MEDLINE
| ID: mdl-28818693
ABSTRACT
Uncovering the relationships between neural activities and capillary-level hemodynamics such as blood flow and concentration of hemoglobin in the brain plays an important role in the study of animal behaviors and brain disorders. Here, we developed a miniature probe integrating a photoacoustic sensor and micro-electrodes to simultaneously record the dynamics of blood flow and total hemoglobin inside a single capillary and the activities of surrounding neurons with high spatiotemporal resolution in freely-moving rats. In the somatosensory cortex of rats, we observed 1) early hemodynamic response prior to the changes in local field potential during pentylenetetrazol-induced localized and generalized seizure onsets in both freely-moving and anesthetized rats; and 2) different hemodynamic and neural responses to generalized seizure onsets between freely-moving and anaesthetized rats. These findings suggest that this high-resolution hybrid technique will enable a wide range of new studies of behaviors and brain disorders in small animals.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Convulsões
/
Córtex Somatossensorial
/
Capilares
/
Acoplamento Neurovascular
/
Hemodinâmica
/
Neurônios
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article