Bacillus wudalianchiensis sp. nov., isolated from grass soils of the Wudalianchi scenic area.
Int J Syst Evol Microbiol
; 67(8): 2897-2902, 2017 Aug.
Article
em En
| MEDLINE
| ID: mdl-28820124
ABSTRACT
A Gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterium, designated FJAT-27215T, was isolated from grass soil collected from Wudalianchi in the Heilongjiang Province of China. Growth was observed at 10-60 °C (optimum 30 °C), in 0 and 3.0â% NaCl (optimum 0â%) and at pH 5.0-10.0 (optimum 7.0), respectively. The cell-wall peptidoglycan contained meso-diaminopimelic acid and the isoprenoid quinone was MK7. The main fatty acids were iso-C15ââ0, anteiso-C15ââ0, anteiso-C17ââ0, and iso-C16ââ0. The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidyl ethanolamine. Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain FJAT-27215T to the genus Bacillus. Strain FJAT-27215T showed high sequence similarities to Bacillus encimensis SGD-V-25T (98.6â%), Bacillus badius NBRC 15713T (98.6â%), Domibacillus indicus SD111T (96.9â%) and Bacillus thermotolerans SgZ-8T (96.5â%). The average nucleotide identity values between strain FJAT-27215T and the type strains of closely related species were much lower than the 96â% threshold value for delineation of genomic prokaryotic species. The in silico DNA-DNA hybridization values between strain FJAT-27215T and the most closely related strain B. encimensis SGD-V-25T showed a similarity of 22.4â% and lower than 70â%, indicating that they belong to different taxa. The phenotypic characters and taxono-genomics study revealed that strain FJAT-27215T represents a novel Bacillus species, for which the name Bacillus wudalianchiensis sp. nov. is proposed. The type strain is FJAT-27215T (=CCTCC AB 2015266T=DSM 100757T).
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Base de dados:
MEDLINE
Assunto principal:
Filogenia
/
Microbiologia do Solo
/
Bacillus
/
Pradaria
País como assunto:
Asia
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article