Mixed Self-Assembly of Polyethylene Glycol and Aptamer on Polydopamine Surface for Highly Sensitive and Low-Fouling Detection of Adenosine Triphosphate in Complex Media.
ACS Appl Mater Interfaces
; 9(36): 31153-31160, 2017 Sep 13.
Article
em En
| MEDLINE
| ID: mdl-28831806
ABSTRACT
Detection of disease biomarkers within complex biological media is a substantial outstanding challenge because of severe biofouling and nonspecific adsorptions. Herein, a reliable strategy for sensitive and low-fouling detection of a biomarker, adenosine triphosphate (ATP) in biological samples was developed through the formation of a mixed self-assembled sensing interface, which was constructed by simultaneously self-assembling polyethylene glycol (PEG) and ATP aptamer onto the self-polymerized polydopamine-modified electrode surface. The developed aptasensor exhibited high selectivity and sensitivity toward the detection of ATP, and the linear range was 0.1-1000 pM, with a detection limit down to 0.1 pM. Moreover, owing to the presence of PEG within the sensing interface, the aptasensor was capable of sensing ATP in complex biological media such as human plasma with significantly reduced nonspecific adsorption effect. Assaying ATP in real biological samples including breast cancer cell lysates further proved the feasibility of this biosensor for practical application.
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Base de dados:
MEDLINE
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article