Release of Enzymatically Active Deubiquitinating Enzymes upon Reversible Capture by Disulfide Ubiquitin Reagents.

de Jong, Annemieke; Witting, Katharina; Kooij, Raymond; Flierman, Dennis; Ovaa, Huib

de Jong, Annemieke; Witting, Katharina; Kooij, Raymond; Flierman, Dennis; Ovaa, Huib.

Afiliação

de Jong A; Department of Cell Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands.
Witting K; Department of Chemical Immunology, Leiden University Medical Center, Einthovenweg 20, 2333 ZC, Leiden, The Netherlands.
Kooij R; Department of Cell Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands.
Flierman D; Department of Chemical Immunology, Leiden University Medical Center, Einthovenweg 20, 2333 ZC, Leiden, The Netherlands.
Ovaa H; Department of Cell Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands.

Angew Chem Int Ed Engl ; 56(42): 12967-12970, 2017 10 09.

Article em En | MEDLINE | ID: mdl-28841265

Assuntos

Enzimas Desubiquitinantes/metabolismo; Dissulfetos/química; Ubiquitina/metabolismo; Biotina/química; Domínio Catalítico; Cisteína/química; Enzimas Desubiquitinantes/química; Dissulfetos/metabolismo; Células HeLa; Humanos; Polietilenoglicóis/química; Proteínas/metabolismo; Ubiquitina/química; Ubiquitinação

Palavras-chave

activity-based protein profiling; disulfide exchange; enzyme activity; post-translational modifications; ubiquitin

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ubiquitina / Dissulfetos / Enzimas Desubiquitinantes Limite: Humans Idioma: En Ano de publicação: 2017 Tipo de documento: Article

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