[Interaction between polycyclic aromatic hydrocarbons and p16, FHIT gene CpG island methylation in patients with cervical intraepithelial neoplasias].
Zhonghua Liu Xing Bing Xue Za Zhi
; 38(8): 1113-1117, 2017 Aug 10.
Article
em Zh
| MEDLINE
| ID: mdl-28847065
ABSTRACT
Objective:
To explore the effect of polycyclic aromatic hydrocarbons (PAHs) and p16, FHIT gene CpG island methylation, as well as their interaction in cervical intraepithelial neoplasias.Methods:
Objects of this study were from a cohort of cervical lesions study in Yangqu county of Shanxi province. All the patients were diagnosed pathologically, that including 83 patients with high-grade cervical intraepithelial neoplasia (CINâ ¡/â ¢), 86 patients with low-grade cervical intraepithelial neoplasia (CINâ ) and another 91 women under normal cervical (NC) condition. 1-hydroxy pyrene in the urine was detected by high performance liquid chromatography (HPLC) while CpG island methylation status of tumor suppressor gene p16 and FHIT were measured by methylation-specifc polymerase chain reaction (MSP). Data were analyzed with Kruskal-Wallis H test, chi-square test and trend of chi-square test. Logistic regression models were used to estimate the odds ratio (OR) and corresponding 95% confidence intervals (95%CI) between influencing factors and the cervical disease by using the SPSS statistical software (version 20.0). The interaction under study was evaluated by using the generalized multifactor dimensionality reduction (GMDR) model.Results:
Level of 1-hydroxy pyrene (H=50.743, P<0.001) and the high exposure rate of 1-hydroxy pyrene (trend χ(2)=20.146, P<0.001) were gradually increasing along with the severity of cervical intraepithelial neoplasia. The CpG island methylation rates of p16, FHIT in CINâ and CINâ ¡/â ¢ group were higher than that in NC group, and gradually increasing along with the severity of cervical intraepithelial neoplasia (trend χ(2)=9.75, P=0.002; trend χ(2)=10.39, P=0.001). Results from the GMDR model showed that interaction existed among the high exposure of 1-hydroxy pyrene and the CpG island methylation of p16, FHIT in CINâ and CINâ ¡/â ¢ group.Conclusion:
Under the high exposure of 1-hydroxy pyrene and the CpG island methylation of p16, FHIT appeared to have increased the risk of cervical intraepithelial neoplasia and causing synergistic effect in cervical intraepithelial neoplasia.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Hidrocarbonetos Policíclicos Aromáticos
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Displasia do Colo do Útero
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Neoplasias do Colo do Útero
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Hidrolases Anidrido Ácido
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Ilhas de CpG
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Papillomavirus Humano 16
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Proteína 2 de Ligação a Metil-CpG
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Proteínas de Neoplasias
Tipo de estudo:
Prognostic_studies
Limite:
Female
/
Humans
Idioma:
Zh
Ano de publicação:
2017
Tipo de documento:
Article