Towards designer organelles by subverting the peroxisomal import pathway.
Nat Commun
; 8(1): 454, 2017 09 06.
Article
em En
| MEDLINE
| ID: mdl-28878206
ABSTRACT
The development of 'designer' organelles could be a key strategy to enable foreign pathways to be efficiently controlled within eukaryotic biotechnology. A fundamental component of any such system will be the implementation of a bespoke protein import pathway that can selectively deliver constituent proteins to the new compartment in the presence of existing endogenous trafficking systems. Here we show that the protein-protein interactions that control the peroxisomal protein import pathway can be manipulated to create a pair of interacting partners that still support protein import in moss cells, but are orthogonal to the naturally occurring pathways. In addition to providing a valuable experimental tool to give new insights into peroxisomal protein import, the variant receptor-signal sequence pair forms the basis of a system in which normal peroxisomal function is downregulated and replaced with an alternative pathway, an essential first step in the creation of a designer organelle.Designer organelles could allow the isolation of synthetic biological pathways from endogenous components of the host cell. Here the authors engineer a peroxisomal protein import pathway orthogonal to the naturally occurring system.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peroxissomos
/
Proteínas de Arabidopsis
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article