Expression of stable and active human DNA topoisomerase I in Pichia pastoris.

Chan, Mooi Kwai; Lim, Shern Kwok; Miswan, Noorizan; Chew, Ai Lan; Noordin, Rahmah; Khoo, Boon Yin

Chan, Mooi Kwai; Lim, Shern Kwok; Miswan, Noorizan; Chew, Ai Lan; Noordin, Rahmah; Khoo, Boon Yin.

Afiliação

Chan MK; Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 Penang, Malaysia.
Lim SK; Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 Penang, Malaysia.
Miswan N; Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 Penang, Malaysia.
Chew AL; Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 Penang, Malaysia.
Noordin R; Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 Penang, Malaysia.
Khoo BY; Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 Penang, Malaysia. Electronic address: boonyin@usm.my.

Protein Expr Purif ; 141: 52-62, 2018 Jan.

Article em En | MEDLINE | ID: mdl-28893606

Assuntos

DNA Topoisomerases Tipo I/metabolismo; Pichia/genética; Proteínas Recombinantes/metabolismo; Linhagem Celular; Clonagem Molecular; DNA Topoisomerases Tipo I/análise; DNA Topoisomerases Tipo I/química; DNA Topoisomerases Tipo I/genética; Humanos; Proteínas Recombinantes/análise; Proteínas Recombinantes/química; Proteínas Recombinantes/genética

Palavras-chave

Cell density; DNA topoisomerase I; MDA-MB-231; Multiple copy integrants; Pichia pastoris; Recombinant protein expression

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Pichia / Proteínas Recombinantes / DNA Topoisomerases Tipo I Limite: Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article

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