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Immobilization on graphene oxide improves the thermal stability and bioconversion efficiency of D-psicose 3-epimerase for rare sugar production.
Dedania, Samir R; Patel, Manisha J; Patel, Dijit M; Akhani, Rekha C; Patel, Darshan H.
Afiliação
  • Dedania SR; Department of Biochemistry, P.D. Patel Institute of Applied Sciences, CHARUSAT, Changa, 388421, Gujarat, India.
  • Patel MJ; Department of Biochemistry, P.D. Patel Institute of Applied Sciences, CHARUSAT, Changa, 388421, Gujarat, India.
  • Patel DM; Department of Advance Organic Chemistry, P.D. Patel Institute of Applied Sciences, CHARUSAT, Changa, 388421, Gujarat, India.
  • Akhani RC; Department of Biochemistry, P.D. Patel Institute of Applied Sciences, CHARUSAT, Changa, 388421, Gujarat, India.
  • Patel DH; Department of Biochemistry, P.D. Patel Institute of Applied Sciences, CHARUSAT, Changa, 388421, Gujarat, India; Dr. K.C. Patel Research and Development Center, CHARUSAT, Changa, 388421, Gujarat, India. Electronic address: darshanpatel.bt@charusat.ac.in.
Enzyme Microb Technol ; 107: 49-56, 2017 Dec.
Article em En | MEDLINE | ID: mdl-28899486
D-Psicose (D-ribo-2-hexulose or D-allulose), an epimer of D-fructose is considered as a rare low-calorie sugar displaying important physiological functions. Enzymatic production using ketose 3-epimerases is the feasible process for the production of D-Psicose. However, major drawbacks in application of ketose 3-epimerases are bioconversion efficiency and reusability of the enzyme. We have attempted immobilization of ketose 3-epimerases from Agrobacterium tumefaciens (agtu) D-psicose 3-epimerase (DPEase) on graphene oxide. Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and Thermo gravimetric analysis (TGA) showed that the enzyme was successfully immobilized on the graphene oxide. Graphene oxide immobilized agtu-DPEase (GO-agtu-DPEase) shows pH optima at 7.5 and 60°C as higher working temperature. Significant improvement in thermal stability was observed which showed half-life of 720min at 60°C whereas Agrobacterium tumefaciens (agtu) DPEase displayed 3.99min. At equilibrium, 40:60 (D-psicose: D-fructose) the bioconversion efficiency was accounted for Graphene oxide immobilized DPEase which is higher than the agtu-DPEase. Graphene oxide immobilized DPEase showed bioconversion efficiency up to 10 cycles of reusability.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Carboidratos Epimerases / Frutose Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Carboidratos Epimerases / Frutose Idioma: En Ano de publicação: 2017 Tipo de documento: Article