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Dosimetric Quantification of Coating-Related Uptake of Silver Nanoparticles.
Lichtenstein, Dajana; Meyer, Thomas; Böhmert, Linda; Juling, Sabine; Fahrenson, Christoph; Selve, Sören; Thünemann, Andreas; Meijer, Jan; Estrela-Lopis, Irina; Braeuning, Albert; Lampen, Alfonso.
Afiliação
  • Lichtenstein D; Department of Food Safety, German Federal Institute for Risk Assessment , Max-Dohrn-Str. 8-10, 10589 Berlin, Germany.
  • Meyer T; Institute for Medical Physics and Biophysics, Leipzig University , Härtelstraße 16-18, 04107 Leipzig, Germany.
  • Böhmert L; Department of Food Safety, German Federal Institute for Risk Assessment , Max-Dohrn-Str. 8-10, 10589 Berlin, Germany.
  • Juling S; Department of Food Safety, German Federal Institute for Risk Assessment , Max-Dohrn-Str. 8-10, 10589 Berlin, Germany.
  • Fahrenson C; ZELMI, Technical University Berlin , Straße des 17. Juni 135, 10623 Berlin, Germany.
  • Selve S; ZELMI, Technical University Berlin , Straße des 17. Juni 135, 10623 Berlin, Germany.
  • Thünemann A; German Federal Institute for Materials Research and Testing , Unter den Eichen 87, 12205 Berlin, Germany.
  • Meijer J; Nuclear Solid State Physics, Leipzig University , Linnéstraße 5, 04103 Leipzig, Germany.
  • Estrela-Lopis I; Institute for Medical Physics and Biophysics, Leipzig University , Härtelstraße 16-18, 04107 Leipzig, Germany.
  • Braeuning A; Department of Food Safety, German Federal Institute for Risk Assessment , Max-Dohrn-Str. 8-10, 10589 Berlin, Germany.
  • Lampen A; Department of Food Safety, German Federal Institute for Risk Assessment , Max-Dohrn-Str. 8-10, 10589 Berlin, Germany.
Langmuir ; 33(45): 13087-13097, 2017 11 14.
Article em En | MEDLINE | ID: mdl-28918629
ABSTRACT
The elucidation of mechanisms underlying the cellular uptake of nanoparticles (NPs) is an important topic in nanotoxicological research. Most studies dealing with silver NP uptake provide only qualitative data about internalization efficiency and do not consider NP-specific dosimetry. Therefore, we performed a comprehensive comparison of the cellular uptake of differently coated silver NPs of comparable size in different human intestinal Caco-2 cell-derived models to cover also the influence of the intestinal mucus barrier and uptake-specialized M-cells. We used a combination of the Transwell system, transmission electron microscopy, atomic absorption spectroscopy, and ion beam microscopy techniques. The computational in vitro sedimentation, diffusion, and dosimetry (ISDD) model was used to determine the effective dose of the particles in vitro based on their individual physicochemical characteristics. Data indicate that silver NPs with a similar size and shape show coating-dependent differences in their uptake into Caco-2 cells. The internalization of silver NPs was enhanced in uptake-specialized M-cells while the mucus did not provide a substantial barrier for NP internalization. ISDD modeling revealed a fivefold underestimation of dose-response relationships of NPs in in vitro assays. In summary, the present study provides dosimetry-adjusted quantitative data about the influence of NP coating materials in cellular uptake into human intestinal cells. Underestimation of particle effects in vitro might be prevented by using dosimetry models and by considering cell models with greater proximity to the in vivo situation, such as the M-cell model.

Texto completo: 1 Base de dados: MEDLINE Tipo de estudo: Prognostic_studies / Qualitative_research Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Tipo de estudo: Prognostic_studies / Qualitative_research Idioma: En Ano de publicação: 2017 Tipo de documento: Article