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A germline-limited piggyBac transposase gene is required for precise excision in Tetrahymena genome rearrangement.
Feng, Lifang; Wang, Guangying; Hamilton, Eileen P; Xiong, Jie; Yan, Guanxiong; Chen, Kai; Chen, Xiao; Dui, Wen; Plemens, Amber; Khadr, Lara; Dhanekula, Arjune; Juma, Mina; Dang, Hung Quang; Kapler, Geoffrey M; Orias, Eduardo; Miao, Wei; Liu, Yifan.
Afiliação
  • Feng L; Department of Pathology, University of Michigan, Ann Arbor, MI 48109, USA.
  • Wang G; Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
  • Hamilton EP; School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China.
  • Xiong J; Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
  • Yan G; Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA 93106, USA.
  • Chen K; Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
  • Chen X; Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
  • Dui W; Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
  • Plemens A; Department of Pathology, University of Michigan, Ann Arbor, MI 48109, USA.
  • Khadr L; Department of Pathology, University of Michigan, Ann Arbor, MI 48109, USA.
  • Dhanekula A; Department of Pathology, University of Michigan, Ann Arbor, MI 48109, USA.
  • Juma M; Department of Pathology, University of Michigan, Ann Arbor, MI 48109, USA.
  • Dang HQ; Department of Pathology, University of Michigan, Ann Arbor, MI 48109, USA.
  • Kapler GM; Department of Pathology, University of Michigan, Ann Arbor, MI 48109, USA.
  • Orias E; Department of Molecular and Cellular Medicine, Texas A&M University Health Science Center, College Station, TX 77843, USA.
  • Miao W; Department of Molecular and Cellular Medicine, Texas A&M University Health Science Center, College Station, TX 77843, USA.
  • Liu Y; Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA 93106, USA.
Nucleic Acids Res ; 45(16): 9481-9502, 2017 Sep 19.
Article em En | MEDLINE | ID: mdl-28934495
ABSTRACT
Developmentally programmed genome rearrangement accompanies differentiation of the silent germline micronucleus into the transcriptionally active somatic macronucleus in the ciliated protozoan Tetrahymena thermophila. Internal eliminated sequences (IES) are excised, followed by rejoining of MAC-destined sequences, while fragmentation occurs at conserved chromosome breakage sequences, generating macronuclear chromosomes. Some macronuclear chromosomes, referred to as non-maintained chromosomes (NMC), are lost soon after differentiation. Large NMC contain genes implicated in development-specific roles. One such gene encodes the domesticated piggyBac transposase TPB6, required for heterochromatin-dependent precise excision of IES residing within exons of functionally important genes. These conserved exonic IES determine alternative transcription products in the developing macronucleus; some even contain free-standing genes. Examples of precise loss of some exonic IES in the micronucleus and retention of others in the macronucleus of related species suggest an evolutionary analogy to introns. Our results reveal that germline-limited sequences can encode genes with specific expression patterns and development-related functions, which may be a recurring theme in eukaryotic organisms experiencing programmed genome rearrangement during germline to soma differentiation.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Protozoários / Tetrahymena thermophila / Transposases Idioma: En Ano de publicação: 2017 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Protozoários / Tetrahymena thermophila / Transposases Idioma: En Ano de publicação: 2017 Tipo de documento: Article