Chromosome Healing Is Promoted by the Telomere Cap Component Hiphop in Drosophila.
Genetics
; 207(3): 949-959, 2017 11.
Article
em En
| MEDLINE
| ID: mdl-28942425
ABSTRACT
The addition of a new telomere onto a chromosome break, a process termed healing, has been studied extensively in organisms that utilize telomerase to maintain their telomeres. In comparison, relatively little is known about how new telomeres are constructed on broken chromosomes in organisms that do not use telomerase. Chromosome healing was studied in somatic and germline cells of Drosophila melanogaster, a nontelomerase species. We observed, for the first time, that broken chromosomes can be healed in somatic cells. In addition, overexpression of the telomere cap component Hiphop increased the survival of somatic cells with broken chromosomes, while the cap component HP1 did not, and overexpression of the cap protein HOAP decreased their survival. In the male germline, Hiphop overexpression greatly increased the transmission of healed chromosomes. These results indicate that Hiphop can stimulate healing of a chromosome break. We suggest that this reflects a unique function of Hiphop it is capable of seeding formation of a new telomeric cap on a chromosome end that lacks a telomere.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Telômero
/
Quebra Cromossômica
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article