Enhanced Mutant Screening in One-step PCR-based Multiple Site-directed Plasmid Mutagenesis by Introduction of Silent Restriction Sites for Structural and Functional Study of Proteins.

Kuo, Ting-Yu; Tsai, Chung-Che; Fu, Hua-Wen

Kuo, Ting-Yu; Tsai, Chung-Che; Fu, Hua-Wen.

Afiliação

Kuo TY; Institute of Molecular and Cellular Biology, National Tsing Hua University, Hsinchu, 30013 Taiwan, Republic of China.
Tsai CC; Institute of Molecular and Cellular Biology, National Tsing Hua University, Hsinchu, 30013 Taiwan, Republic of China.
Fu HW; Institute of Molecular and Cellular Biology, National Tsing Hua University, Hsinchu, 30013 Taiwan, Republic of China.

Biol Proced Online ; 19: 12, 2017.

Article em En | MEDLINE | ID: mdl-28959142

Palavras-chave

Helicobacter pylori neutrophil-activating protein, HP-NAP; PCR; Polymerase chain reaction; Silent restriction mutation; Site-directed mutagenesis

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Texto completo: 1 Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Screening_studies Idioma: En Ano de publicação: 2017 Tipo de documento: Article

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Texto completo: 1 Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Screening_studies Idioma: En Ano de publicação: 2017 Tipo de documento: Article