Effects of 4-nonylphenol on oxidant/antioxidant balance system inducing hepatic steatosis in male rat.
Toxicol Rep
; 2: 1423-1433, 2015.
Article
em En
| MEDLINE
| ID: mdl-28962484
ABSTRACT
An emerging literature suggests that early life exposure to 4-nonylphenol (4-NP), a widespread endocrine disrupting chemical, may increase the risk of metabolic syndrome. In this study, we investigated the hypothesis that intraperitoneal administration of 4-NP induces hepatic steatosis in rat. 24 male Sprague-Dawley rats were administered with 4-NP (0, 2, 10 and 50 mg/kg b.wt) in corn oil for 30 days. Liver histology, biochemical analysis and gene expression profiling were examined. After treatment, abnormal liver morphology and function were observed in the 4-NP-treated rat, and significant changes in gene expression an indicator of hepatic steatosis and apoptosis were observed compared with controls. Up-regulated genes involved in apoptosis, hepatotoxity and oxidative stress, increased ROS and decrease of antioxidant enzyme were observed in the 4-NP exposed rat. Extensive fatty accumulation in liver section and elevated serum GOT, GPT, LDH and γ-GT were also observed. Incidence and severity of liver steatosis was scored and taken into consideration (steatosis, ballooning and lobular inflammation). Hepatocytes apoptosis could promote NAFLD progression; Fas/FasL, TNF-α and Caspase-9 mRNA activation were important contributing factors to hepatic steatosis. These findings provide the first evidence that 4-NP affects the gene expression related to liver hepatotoxicity, which is correlated with hepatic steatosis.
4-NP, 4-nonylphenol; 4-Nonylphenol; 4-Nonylphenol (PubChem CID: 1752); APNEIs, alkylphenol polyethoxylates; AhR, aril hydrocarbon receptor; Apoptosis; Aprotinin (PubChem CID: 22833874); Bouin's fluid (PubChem CID: 124013); Collagenase (PubChem CID: 5046512); Cyt c, cytochrome c; Diamninobenzidine Tetrahydrochloride (PubChem CID: 23892); FAO, fatty acid oxidation; FFA, free fatty acid; GOT, glutamic-oxalacetic transaminase; GPT, glutamate pyruvate transaminase; Genes; HSC, hepatic stellate cell; Hematoxylin Eosin (PubChem CID: 86598188); Hepatic steatosis; Hydrogen peroxide (PubChem CID: 784); IR, insulin resistance; LDH, lactate dehydrogenase; Liver; Malondialdehyde (PubChem CID: 10964); NAFLD, nonalcoholic fatty liver disease; NASH, non-alcoholic hepatic steatosis; Nitrotetrazolium Blue chloride (PubChem CID: 9281); OS, oxidative stress; Oxidative stress; PPAR, peroxisome proliferation-activated receptor; Phenylmethylsulfonyl fluoride (PubChem CID: 4784); ROS, reactive oxygen species; Sodium chloride (PubChem CID: 5234); Superoxide (PubChem CID: 5359597); TAG, triacylglycerol; Thiobarbituric Acid (PubChem CID: 2723628); Trizol (PubChem CID: 378478); Tromethamine (Tris) (PubChem CID: 6503); Xylene (PubChem CID: 6850715); γ-GT, gamma glutamyltransferase
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Base de dados:
MEDLINE
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article