CRISPR/Cas9-induced transgene insertion and telomere-associated truncation of a single human chromosome for chromosome engineering in CHO and A9 cells.

Uno, Narumi; Hiramatsu, Kei; Uno, Katsuhiro; Komoto, Shinya; Kazuki, Yasuhiro; Oshimura, Mitsuo

Uno, Narumi; Hiramatsu, Kei; Uno, Katsuhiro; Komoto, Shinya; Kazuki, Yasuhiro; Oshimura, Mitsuo.

Afiliação

Uno N; Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan.
Hiramatsu K; Chromosome Engineering Research Center, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan.
Uno K; Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan.
Komoto S; Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan.
Kazuki Y; Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan.
Oshimura M; Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan.

Sci Rep ; 7(1): 12739, 2017 10 06.

Article em En | MEDLINE | ID: mdl-28986519

Assuntos

Proteína 9 Associada à CRISPR/metabolismo; Sistemas CRISPR-Cas/genética; Cromossomos Humanos/genética; Engenharia Genética; Mutagênese Insercional/genética; Transgenes; Animais; Células CHO; Linhagem Celular Tumoral; Cricetinae; Cricetulus; Marcação de Genes; Vetores Genéticos/metabolismo; Humanos; Melanoma Experimental/patologia; Camundongos; Plasmídeos/genética; Telômero

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Engenharia Genética / Mutagênese Insercional / Cromossomos Humanos / Transgenes / Sistemas CRISPR-Cas / Proteína 9 Associada à CRISPR Tipo de estudo: Risk_factors_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2017 Tipo de documento: Article

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