Improved process conditions for increasing expression of MHC class II protein from a stable Drosophila S2 cell line.

Shen, Xiao; Dojcinovic, Danijel; Baldi, Lucia; Hacker, David L; Luescher, Immanuel F; Wurm, Florian M

Shen, Xiao; Dojcinovic, Danijel; Baldi, Lucia; Hacker, David L; Luescher, Immanuel F; Wurm, Florian M.

Afiliação

Shen X; Laboratory of Cellular Biotechnology, Faculty of Life Sciences, École Polytechnique Fédérale de Lausanne, 1015, Lausanne, Switzerland. xiao.shen@cantonbio.com.
Dojcinovic D; R&D Department, Cantonbio Co., Ltd, Guangzhou, 510200, China. xiao.shen@cantonbio.com.
Baldi L; Molecular Immunology Group, Ludwig Center for Cancer Research, University of Lausanne, 1066, Epalinges, Switzerland.
Hacker DL; TCMetrix Ltd, 1066, Epalinges, Switzerland.
Luescher IF; Covance Central Laboratory Services SA, 1217, Meyrin, Switzerland.
Wurm FM; Laboratory of Cellular Biotechnology, Faculty of Life Sciences, École Polytechnique Fédérale de Lausanne, 1015, Lausanne, Switzerland.

Biotechnol Lett ; 40(1): 85-92, 2018 Jan.

Article em En | MEDLINE | ID: mdl-28993910

Assuntos

Técnicas de Cultura de Células/métodos; Antígeno HLA-DR1/biossíntese; Proteínas Recombinantes/biossíntese; Animais; Reatores Biológicos; Biotecnologia/métodos; Linhagem Celular; Proliferação de Células; Drosophila; Antígeno HLA-DR1/genética; Proteínas Recombinantes/genética

Palavras-chave

Orbital shaking; Recombinant protein; Roller bottle; S2 cells; Tetramer technology

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas Recombinantes / Antígeno HLA-DR1 / Técnicas de Cultura de Células Limite: Animals Idioma: En Ano de publicação: 2018 Tipo de documento: Article

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