Regulation of nonylphenol-induced reproductive toxicity in mouse spermatogonia cells by miR-361-3p.
Mol Reprod Dev
; 84(12): 1257-1270, 2017 12.
Article
em En
| MEDLINE
| ID: mdl-29024157
ABSTRACT
Nonylphenol (NP) is an environmental chemical that affects apoptosis and male infertility. In our study, we found that a high concentration of NP could down-regulate the expression of microRNA-361-3p (miR-361-3p) in the murine GC-1 spermatogonia cell line and in vivo in murine spermatogonia. Additionally, one direct target of this miR, the 3' untranslated region of Killin (Klln) mRNA, was identified. Klln encodes a transcription factor that directly regulates the expression of Tp73 (transcriptionally active p73), whose encoded protein can up-regulate the expression of Puma (p53 upregulated modulator of apoptosis). Thus, our investigation revealed that the expression of Klln, Tp73, and Puma increased upon NP-dependent down-regulation of miR-361-3p, which eventually leads to apoptosis of spermatogonia.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fenóis
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Espermatogônias
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Regulação para Baixo
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Apoptose
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MicroRNAs
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article