[Inhibitory mechanisms of timosaponin Aâ
¢ on proliferation and growth of human glioblastoma cells].
Zhongguo Zhong Yao Za Zhi
; 42(6): 1160-1166, 2017 Mar.
Article
em Zh
| MEDLINE
| ID: mdl-29027433
ABSTRACT
To explore the inhibitory effect of timosaponin Aâ
¢ on the proliferation of human glioblastoma cell line U87MG and investigate its related mechanism. As compared with the model group, the tumor weight was significantly reduced in timosaponin Aâ
¢-treated group. Timosaponin Aâ
¢inhibited the proliferation of U87MG cell line in a dose-dependent manner. It up-regulated the gene and protein expression levels of p21, meanwhile inhibited the protein expression levels of ß-Catenin, Cyclin D1 and Bcl-2. It also inhibited the translocation of ß-Catenin into nucleus, suppressed the phosphorylation expression of ERK, but increased the phosphorylation expression of p38 and JNK. Combined use of JNK inhibitor SP600125 and p38 inhibitor SB203580 could decrease p21 and increase ß-Catenin protein expressions. Timosaponin Aâ
¢ inhibited the proliferation of human glioblastoma cell line U87MG partly by intervening MAPK and Wnt/ß-Catenin signal pathways.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Saponinas
/
Esteroides
/
Glioblastoma
/
Proliferação de Células
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2017
Tipo de documento:
Article