Productive replication of avian influenza viruses in chicken endothelial cells is determined by hemagglutinin cleavability and is related to innate immune escape.
Virology
; 513: 29-42, 2018 01 01.
Article
em En
| MEDLINE
| ID: mdl-29031164
ABSTRACT
Endotheliotropism is a hallmark of gallinaceous poultry infections with highly pathogenic avian influenza (HPAI) viruses and a feature that distinguishes HPAI from low pathogenic avian influenza (LPAI) viruses. Here, we used chicken aortic endothelial cells (chAEC) as a novel in vitro infection model to assess the susceptibility, permissiveness, and host response of chicken endothelial cells (EC) to infections with avian influenza (AI) viruses. Our data show that productive replication of AI viruses in chAEC is critically determined by hemagglutinin cleavability, and is thus an exclusive trait of HPAI viruses. However, we provide evidence for a link between limited (i.e. trypsin-dependent) replication of certain LPAI viruses, and the viruses' ability to dampen the antiviral innate immune response in infected chAEC. Strikingly, this cell response pattern was also detected in HPAI virus-infected chAEC, suggesting that viral innate immune escape might be a prerequisite for robust AI virus replication in chicken EC.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Vírus da Influenza A
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Replicação Viral
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Glicoproteínas de Hemaglutininação de Vírus da Influenza
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Células Endoteliais
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Internalização do Vírus
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Evasão da Resposta Imune
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Imunidade Inata
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article