Analysis of selected aspects of inflammasome function in the monocytes from neonates born extremely and very prematurely.
Immunobiology
; 223(1): 18-24, 2018 01.
Article
em En
| MEDLINE
| ID: mdl-29042061
ABSTRACT
BACKGROUND:
Inflammasomes regulate activation of caspase-1, which cleaves and activates interleukin (IL)-1ß and IL-18, the cytokines that trigger pro-inflammatory and antimicrobial responses. There is very little known about inflammasome function in the subsets of monocytes (MO) isolated from preterm neonates born extremely and very prematurely.METHODS:
A group of 76 very low birth weight patients without early-onset sepsis was divided into extremely preterm (<28 gestational week) or very preterm (28-32 gestational week) neonates. The first blood sample was collected on the 5th day of life (5th DOL) to analyse MO subsets as well as the intracellular IL-1ß expression and supernatant concentration of IL-1ß and IL-18. Secondary blood samples were collected within 24h of late-onset sepsis (LOS) development and analysed as above.RESULTS:
On the 5th DOL, the extremely preterm neonates were characterized by a significantly higher absolute count of MO, in particular in the classical and intermediate subsets, as compared to the very preterm group. The counts of the intermediate and non-classical MO subsets increased during LOS in all neonates. We did not observe significant differences in the intracellular IL-1ß expression between the analysed groups. Furthermore, the levels of the analysed cytokines in the MO supernatants were comparable between the extremely and very preterm neonates on the 5th DOL. Finally, a higher level of IL-18 was observed in the supernatant of the extremely preterm group during LOS.CONCLUSIONS:
During LOS, extremely preterm neonates excrete a higher level of IL-18 cytokines compared to very preterm neonates. Further studies are required to determine whether this observation is a result of a higher count of the circulating MO or is a true reflection of increased inflammasome function in this particular group of newborns.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Monócitos
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Sepse
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Interleucina-18
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Nascimento Prematuro
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Inflamassomos
Tipo de estudo:
Diagnostic_studies
Limite:
Female
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Humans
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Male
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Newborn
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article