Recruitment of 7SL RNA to assembling HIV-1 virus-like particles.
Traffic
; 19(1): 36-43, 2018 01.
Article
em En
| MEDLINE
| ID: mdl-29044909
ABSTRACT
Retroviruses incorporate specific host cell RNAs into virions. In particular, the host noncoding 7SL RNA is highly abundant in all examined retroviruses compared with its cellular levels or relative to common mRNAs such as actin. Using live cell imaging techniques, we have determined that the 7SL RNA does not arrive with the HIV-1 RNA genome. Instead, it is recruited contemporaneously with assembly of the protein HIV-1 Gag at the plasma membrane. Further, we demonstrate that complexes of 7SL RNA and Gag can be immunoprecipitated from both cytosolic and plasma membrane fractions. This indicates that 7SL RNAs likely interact with Gag prior to high-order Gag multimerization at the plasma membrane. Thus, the interactions between Gag and the host RNA 7SL occur independent of the interactions between Gag and the host endosomal sorting complex required for transport (ESCRT) proteins, which are recruited temporarily at late stages of assembly. The interactions of 7SL and Gag are also independent of interactions of Gag and the HIV-1 genome which are seen on the plasma membrane prior to assembly of Gag.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
HIV-1
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Partícula de Reconhecimento de Sinal
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Montagem de Vírus
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RNA Citoplasmático Pequeno
Limite:
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article