Your browser doesn't support javascript.
loading
Genome structure of Rosa multiflora, a wild ancestor of cultivated roses.
Nakamura, Noriko; Hirakawa, Hideki; Sato, Shusei; Otagaki, Shungo; Matsumoto, Shogo; Tabata, Satoshi; Tanaka, Yoshikazu.
Afiliação
  • Nakamura N; Suntory Global Innovation Center Ltd, Seika-cho, Soraku-gun, Kyoto 619-0284, Japan.
  • Hirakawa H; Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.
  • Sato S; Graduate School of Life Sciences, Tohoku University, Aoba-ku, Sendai, Miyagi 980-8577, Japan.
  • Otagaki S; Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan.
  • Matsumoto S; Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan.
  • Tabata S; Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.
  • Tanaka Y; Suntory Global Innovation Center Ltd, Seika-cho, Soraku-gun, Kyoto 619-0284, Japan.
DNA Res ; 25(2): 113-121, 2018 Apr 01.
Article em En | MEDLINE | ID: mdl-29045613
ABSTRACT
The draft genome sequence of a wild rose (Rosa multiflora Thunb.) was determined using Illumina MiSeq and HiSeq platforms. The total length of the scaffolds was 739,637,845 bp, consisting of 83,189 scaffolds, which was close to the 711 Mbp length estimated by k-mer analysis. N50 length of the scaffolds was 90,830 bp, and extent of the longest was 1,133,259 bp. The average GC content of the scaffolds was 38.9%. After gene prediction, 67,380 candidates exhibiting sequence homology to known genes and domains were extracted, which included complete and partial gene structures. This large number of genes for a diploid plant may reflect heterogeneity of the genome originating from self-incompatibility in R. multiflora. According to CEGMA analysis, 91.9% and 98.0% of the core eukaryotic genes were completely and partially conserved in the scaffolds, respectively. Genes presumably involved in flower color, scent and flowering are assigned. The results of this study will serve as a valuable resource for fundamental and applied research in the rose, including breeding and phylogenetic study of cultivated roses.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Análise de Sequência de RNA / Análise de Sequência de DNA / Genoma de Planta / Regulação da Expressão Gênica de Plantas / Polimorfismo de Nucleotídeo Único / Rosa Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Análise de Sequência de RNA / Análise de Sequência de DNA / Genoma de Planta / Regulação da Expressão Gênica de Plantas / Polimorfismo de Nucleotídeo Único / Rosa Idioma: En Ano de publicação: 2018 Tipo de documento: Article